Predicting Rubisco-Linker Condensation from Titration in the Dilute Phase
Published in arXiv, 2023
The condensation of Rubisco holoenzymes and linker proteins into “pyrenoids,” a crucial supercharger of photosynthesis in algae, is qualitatively understood in terms of “sticker-and-spacer” theory. We derive semianalytical partition sums for small Rubisco-linker aggregates, which enable the calculation of both dilute-phase titration curves and dimerization diagrams. By fitting the titration curves to surface plasmon resonance and single-molecule fluorescence microscopy data, we extract the molecular properties needed to predict dimerization diagrams. We use these to estimate typical concentrations for condensation, and successfully compare these to microscopy observations.
Cite: Payne-Dwyer et al., "Predicting Rubisco-Linker Condensation from Titration in the Dilute Phase." Phys. Rev. Lett. 132</i>, 218401 (2023) [https://arxiv.org/abs/2301.05681](https://journals.aps.org/prl/abstract/10.1103/PhysRevLett.132.218401)</p> </article> </div>
Sticker-and-Spacer Model for Amyloid Beta Condensation and Fibrillation
Published in [Frontiers in Molecular Neuroscience](https://doi.org/10.3389/fnmol.2022.962526), 2022
Amyloid-Beta Aggregation
Cite: Jack Connor, Steve Quinn, Charley Schaefer, "Sticker-and-Linker Model for Amyloid Beta Condensation and Fibrillation." Frontiers in Molecular Neuroscience. 15, 962526 (2022)
Theoretical rheo-physics of silk: Intermolecular associations reduce the critical specific work for flow-induced crystallisation
Published in [J. Rheol](https://sor.scitation.org/doi/full/10.1122/8.0000411), 2021
Theoretical rheo-physics of silk: Intermolecular associations reduce the critical specific work for flow-induced crystallisation
Cite: Charley Schaefer & Tom C. B. McLeish, "Theoretical rheo-physics of silk: Intermolecular associations reduce the critical specific work for flow-induced crystallisation." arXiv.. (2021)
Membraneless organelles formed by liquid-liquid phase separation increase bacterial fitness
Published in Science Advances, 2021
Liquid-liquid phase separation of proteins to form membraneless organelles in living bacteria.
Cite: Xin Jin, Ji-Eun Lee, Charley Schaefer, Xinwei Luo, Adam J. M. Wollman, Alex L. Payne-Dwyer, Tian Tian, Xiaowei Zhang, Xiao Chen, Yingxing Li, Tom C. B. McLeish, Mark C. Leake, Fan Bai. "Membraneless organelles formed by liquid-liquid phase separation increase bacterial fitness." Science Adv.. 7, 43 (2021) https://www.science.org/doi/10.1126/sciadv.abh2929
Power Law Stretching of Associating Polymers in Steady-State Extensional Flow
Published in Physical Review Letters, 2021
Polymer physics for the linear viscoelastic response of silk feedstock.
Cite: C. Schaefer & T.C.B. McLeish. "Power Law Stretching of Associating Polymers in Steady-State Extensional Flow." Phys. Rev. Lett.. 126, 057801 (2021) https://journals.aps.org/prl/abstract/10.1103/PhysRevLett.126.057801
Silk Protein Solution: A Natural Example of Sticky Reptation
Published in Macromolecules, 2020
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Cite: C. Schaefer, P.R. Laity, C. Holland, T.C.B. McLeish. "Silk Protein Solution: A Natural Example of Sticky Reptation." Macromolecules. 53, 2669–2676 (2020) https://pubs.acs.org/doi/abs/10.1021/acs.macromol.9b02630
Morphology formation in binary mixtures upon gradual destabilisation
Published in Soft Matter, 2019
Impact of thermal fluctuations on phase separation during a continuous quench.
Cite: C. Schaefer, S. Paquay, Tom C.B. McLeish. "Morphology formation in binary mixtures upon gradual destabilisation." Soft Matter. Soft Matter, 15, 8450-8458 (2019) https://doi.org/10.1039/C9SM01344J
Ligand-regulated oligomerisation of allosterically interacting proteins
Published in Soft Matter, 2018
Impact of dynamic allostery on supramolecular polymerisation.
Cite: C. Schaefer, R. A. J. de Bruijn, T. C. B. McLeish. "Ligand-regulated oligomerisation of allosterically interacting proteins." Soft Matter. 14, 6961-6968 (2018) https://doi.org/10.1039/C8SM00943K
Structuring of Fluid Adlayers upon Ongoing Unimolecular Adsorption
Published in Physical Review Letters, 2018
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Cite: C. Schaefer. " Structuring of Fluid Adlayers upon Ongoing Unimolecular Adsorption. " Phys. Rev. Lett.. 120, 036001 (2018) https://journals.aps.org/prl/abstract/10.1103/PhysRevLett.120.036001
Dynamic Surface Enrichment in Drying Thin-Film Binary Polymer Solutions
Published in Macromolecules, 2017
Manufacturing of Organic Solar Cells.
Cite: C. Schaefer, J.J. Michels, P. van der Schoot. "Dynamic Surface Enrichment in Drying Thin-Film Binary Polymer Solutions." Macromolecules. 50, 5914-5919 (2017) https://pubs.acs.org/doi/10.1021/acs.macromol.7b01224
Structuring of Thin-Film Polymer Mixtures upon Solvent Evaporation
Published in Macromolecules, 2016
Manufacturing of Organic Solar Cells.
Cite: C. Schaefer, J.J. Michels, P. van der Schoot. "Structuring of Thin-Film Polymer Mixtures upon Solvent Evaporation." Macromolecules. 49, 6858–6870 (2016) https://pubs.acs.org/doi/abs/10.1021/acs.macromol.6b00537
Polymer Solar Cells: Solubility Controls Fiber Network Formation
Published in J. Am. Chem. Soc., 2015
We found that the influence of the solvent quality on the polymer fibre size can be understood in terms of classical nucleation theory.
Cite: J. J. van Franeker, G. H. L. Heintges, C. Schaefer, G. Portale, W. Li, M. M. Wienk, P. van der Schoot, R. A. J. Janssen." Polymer Solar Cells: Solubility Controls Fiber Network Formation." J. Am. Chem. Soc.. 137, 11783-11794 (2015) https://pubs.acs.org/doi/abs/10.1021/mz300218e
Coupling of kinetic Monte Carlo simulations of surface reactions to transport in a fluid for heterogeneous catalytic reactor modeling
Published in J. Chem. Phys., 2013
Kinetic Monte Carlo simulations of surface-catalysed chemical reactions
Cite: C. Schaefer and A.P.J. Jansen ." Coupling of kinetic Monte Carlo simulations of surface reactions to transport in a fluid for heterogeneous catalytic reactor modeling. " J. Chem. Phys. 138, 054102 (2013) https://aip.scitation.org/doi/10.1063/1.4789419
Controlling Chemical Self-Assembly by Solvent-Dependent Dynamics
Published in J. Am. Chem. Soc., 2012
We showed how the solvent quality affects the dynamics of supramolecular polymerisation.
Cite: P.A. Korevaar, C. Schaefer, T.F.A. de Greef, E.W. Meijer ." Controlling Chemical Self-Assembly by Solvent-Dependent Dynamics. " J. Am. Chem. Soc.. 134, 13482-13491 (2012) https://pubs.acs.org/doi/abs/10.1021/mz300218e
Controlling the Cooperativity in the Supramolecular Polymerization of Ionic Discotic Amphiphiles via Electrostatic Screening
Published in ACS Macro Letters, 2012
Controlling supramolecular polymerisation using electrostatic screening.
Cite: C. Schaefer, I. K. Voets, A. R. A. Palmans, E. W. Meijer, P. van der Schoot, P. Besenius. "Ligand-regulated oligomerisation of allosterically interacting proteins." ACS Macro Lett.. 1, 830-833 (2012) https://pubs.acs.org/doi/abs/10.1021/mz300218e