Predicting Rubisco-Linker Condensation from Titration in the Dilute Phase
Published in arXiv, 2023
The condensation of Rubisco holoenzymes and linker proteins into “pyrenoids,” a crucial supercharger of photosynthesis in algae, is qualitatively understood in terms of “sticker-and-spacer” theory. We derive semianalytical partition sums for small Rubisco-linker aggregates, which enable the calculation of both dilute-phase titration curves and dimerization diagrams. By fitting the titration curves to surface plasmon resonance and single-molecule fluorescence microscopy data, we extract the molecular properties needed to predict dimerization diagrams. We use these to estimate typical concentrations for condensation, and successfully compare these to microscopy observations.
Cite: Payne-Dwyer et al., "Predicting Rubisco-Linker Condensation from Titration in the Dilute Phase." Phys. Rev. Lett. 132</i>, 218401 (2023) [https://arxiv.org/abs/2301.05681](https://journals.aps.org/prl/abstract/10.1103/PhysRevLett.132.218401)</p> </article> </div> Published in [Frontiers in Molecular Neuroscience](https://doi.org/10.3389/fnmol.2022.962526), 2022 Cite: Jack Connor, Steve Quinn, Charley Schaefer, "Sticker-and-Linker Model for Amyloid Beta Condensation and Fibrillation." Frontiers in Molecular Neuroscience. 15, 962526 (2022) Published in [J. Rheol](https://sor.scitation.org/doi/full/10.1122/8.0000411), 2021 Cite: Charley Schaefer & Tom C. B. McLeish, "Theoretical rheo-physics of silk: Intermolecular associations reduce the critical specific work for flow-induced crystallisation." arXiv.. (2021) Published in Science Advances, 2021 Cite: Xin Jin, Ji-Eun Lee, Charley Schaefer, Xinwei Luo, Adam J. M. Wollman, Alex L. Payne-Dwyer, Tian Tian, Xiaowei Zhang, Xiao Chen, Yingxing Li, Tom C. B. McLeish, Mark C. Leake, Fan Bai. "Membraneless organelles formed by liquid-liquid phase separation increase bacterial fitness." Science Adv.. 7, 43 (2021) https://www.science.org/doi/10.1126/sciadv.abh2929 Published in Physical Review Letters, 2021 Cite: C. Schaefer & T.C.B. McLeish. "Power Law Stretching of Associating Polymers in Steady-State Extensional Flow." Phys. Rev. Lett.. 126, 057801 (2021) https://journals.aps.org/prl/abstract/10.1103/PhysRevLett.126.057801 Published in Macromolecules, 2020 Click here for our follow-up research. Cite: C. Schaefer, P.R. Laity, C. Holland, T.C.B. McLeish. "Silk Protein Solution: A Natural Example of Sticky Reptation." Macromolecules. 53, 2669–2676 (2020) https://pubs.acs.org/doi/abs/10.1021/acs.macromol.9b02630 Published in Soft Matter, 2019 Cite: C. Schaefer, S. Paquay, Tom C.B. McLeish. "Morphology formation in binary mixtures upon gradual destabilisation." Soft Matter. Soft Matter, 15, 8450-8458 (2019) https://doi.org/10.1039/C9SM01344J Published in Soft Matter, 2018 Cite: C. Schaefer, R. A. J. de Bruijn, T. C. B. McLeish. "Ligand-regulated oligomerisation of allosterically interacting proteins." Soft Matter. 14, 6961-6968 (2018) https://doi.org/10.1039/C8SM00943K Published in Physical Review Letters, 2018 Click here for follow-up research. Cite: C. Schaefer. " Structuring of Fluid Adlayers upon Ongoing Unimolecular Adsorption. " Phys. Rev. Lett.. 120, 036001 (2018) https://journals.aps.org/prl/abstract/10.1103/PhysRevLett.120.036001 Published in Macromolecules, 2017 Cite: C. Schaefer, J.J. Michels, P. van der Schoot. "Dynamic Surface Enrichment in Drying Thin-Film Binary Polymer Solutions." Macromolecules. 50, 5914-5919 (2017) https://pubs.acs.org/doi/10.1021/acs.macromol.7b01224 Published in Macromolecules, 2016 Manufacturing of Organic Solar Cells. Cite: C. Schaefer, J.J. Michels, P. van der Schoot. "Structuring of Thin-Film Polymer Mixtures upon Solvent Evaporation." Macromolecules. 49, 6858–6870 (2016) https://pubs.acs.org/doi/abs/10.1021/acs.macromol.6b00537 Published in J. Am. Chem. Soc., 2015 Cite: J. J. van Franeker, G. H. L. Heintges, C. Schaefer, G. Portale, W. Li, M. M. Wienk, P. van der Schoot, R. A. J. Janssen." Polymer Solar Cells: Solubility Controls Fiber Network Formation." J. Am. Chem. Soc.. 137, 11783-11794 (2015) https://pubs.acs.org/doi/abs/10.1021/mz300218e Published in J. Chem. Phys., 2013 Kinetic Monte Carlo simulations of surface-catalysed chemical reactions Cite: C. Schaefer and A.P.J. Jansen ." Coupling of kinetic Monte Carlo simulations of surface reactions to transport in a fluid for heterogeneous catalytic reactor modeling. " J. Chem. Phys. 138, 054102 (2013) https://aip.scitation.org/doi/10.1063/1.4789419 Published in J. Am. Chem. Soc., 2012 Cite: P.A. Korevaar, C. Schaefer, T.F.A. de Greef, E.W. Meijer ." Controlling Chemical Self-Assembly by Solvent-Dependent Dynamics. " J. Am. Chem. Soc.. 134, 13482-13491 (2012) https://pubs.acs.org/doi/abs/10.1021/mz300218e Published in ACS Macro Letters, 2012 Cite: C. Schaefer, I. K. Voets, A. R. A. Palmans, E. W. Meijer, P. van der Schoot, P. Besenius. "Ligand-regulated oligomerisation of allosterically interacting proteins." ACS Macro Lett.. 1, 830-833 (2012) https://pubs.acs.org/doi/abs/10.1021/mz300218e Sticker-and-Spacer Model for Amyloid Beta Condensation and Fibrillation
Amyloid-Beta Aggregation Theoretical rheo-physics of silk: Intermolecular associations reduce the critical specific work for flow-induced crystallisation
Theoretical rheo-physics of silk: Intermolecular associations reduce the critical specific work for flow-induced crystallisation Membraneless organelles formed by liquid-liquid phase separation increase bacterial fitness
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Morphology formation in binary mixtures upon gradual destabilisation
Impact of thermal fluctuations on phase separation during a continuous quench. Ligand-regulated oligomerisation of allosterically interacting proteins
Impact of dynamic allostery on supramolecular polymerisation. Structuring of Fluid Adlayers upon Ongoing Unimolecular Adsorption
Dynamic Surface Enrichment in Drying Thin-Film Binary Polymer Solutions
Manufacturing of Organic Solar Cells. Structuring of Thin-Film Polymer Mixtures upon Solvent Evaporation
Polymer Solar Cells: Solubility Controls Fiber Network Formation
We found that the influence of the solvent quality on the polymer fibre size can be understood in terms of classical nucleation theory. Coupling of kinetic Monte Carlo simulations of surface reactions to transport in a fluid for heterogeneous catalytic reactor modeling
Controlling Chemical Self-Assembly by Solvent-Dependent Dynamics
We showed how the solvent quality affects the dynamics of supramolecular polymerisation. Controlling the Cooperativity in the Supramolecular Polymerization of Ionic Discotic Amphiphiles via Electrostatic Screening
Controlling supramolecular polymerisation using electrostatic screening.